25 Slides/50 Grid Cover Slips

Hematology Testing Directions For Use

Cell-VU ® CBC is a disposable hemacytometer used for manual cell counting. It consists of a dual-chamber glass slide, patterned from a printed inert surface. This surface supports two cover slips, each containing a laser-etched grid on the reverse side of the cover slip. Two tests can be performed with each Cell-VU® slide.

The Cell-VU® CBC grid pattern is a modification of the Neubauer Ruling. It consists of 9 large squares, each measuring 1 * 1 mm, giving a total area of 3 * 3 mm or or 9 sq mm of counting area. The depth of the Cell-VU® cham-ber is 0.02 mm, giving a total grid volume for each chamber of 0.18 cu mm.

The four corner squares are divided into 16 smaller squares (each 1/16 sq mm in area).The central square, used prima-rily for erythrocyte, platelet and sperm counts, is divided into 100 small squares (each 0.1 by 0.1 mm). The perimeter circle on the cover glass surrounds the Cell-VU CBC ruling. The total volume of this area is 0.5 cu mm.

This area is primarily used for cell counts from low cellularity body fluids such as spinal fluid.

Mix the sample thoroughly just before pipetting.

Place one cover glass onto the slide as shown in Figures A and B.

The cover glass should cover approximately three quarters of one of the sampling areas, as shown in Figure B.

(Ensure the Cell-VU® name on the cover glass is facing the observer as the grid is etched on the reverse side.) Pipette one drop (approximately 4 microliters) of specimen.

Pipette the specimen into the sampling area so that it fills the sampling area by capillary action as shown in Figure C.

Move the cover glass into final position so that the circle on the cover glass is centered within the circle of the sampling area (Figures D and E).

A. Erythrocyte count (1:200 dilution)
Dilute blood using accepted laboratory methods. Disposable dilution preparations such as the UNOPETTE® system #5850 or #5851 can also be used. Pipette diluted specimen onto the Cell-VU® CBC slide according to the directions in general preparation. Allow cells to settle for 1 to 3 minutes before counting. Isolate and view the Cell-VU® CBC ruling.

Count the erythrocytes in the large center square of the Cell-VU® CBC ruling (this center square is divided into 100 smaller squares). Multiply the cells counted by 10,000 to obtain the total erythrocytes/cu mm.

B. Leukocyte count (1:20 dilution)
Dilute blood using accepted laboratory methods. Disposable dilution preparations such as the UNOPETTE® system #5856 can also be used.

Pipette diluted specimen onto the Cell-VU® CBC slide according to the directions in general preparation. Allow cells to settle for 1 to 3 minutes before counting. Isolate and view the Cell-VU® CBC ruling.

Count the leukocytes using 100x magnification. Count leukocytes in all 9 squares of the Cell-VU® CBC ruling. Multiply the number of leukocytes counted by 111 to obtain the total number of leukocytes/cu mm.

C. Leukocyte count (1:100 dilution)
Dilute blood using accepted laboratory methods. Disposable dilution preparations such as the UNOPETTE® system #5804, #5853, #5854, #5855 can also be used.

Pipette diluted specimen onto the Cell-VU® CBC slide according to the directions in general preparation. Allow cells to settle for 1 to 3 minutes before counting. Isolate and view the Cell-VU® CBC ruling.

Count the leukocytes using 100x magnification. Count leukocytes in all 9 squares of the Cell-VU® CBC ruling. Multiply the total number of leukocytes counted by 555 to obtain the total leukocytes / mm.

D. Platelet count (1:100 dilution)
Dilute blood using accepted laboratory methods. Disposable dilution preparations such as the UNOPETTE® system #5854, #5855 can also be used.

Pipette diluted specimen onto the Cell-VU® CBC slide according to the directions in general preparation. Allow cells to settle for 10 minutes before counting. Isolate and view the Cell-VU® CBC ruling. Using a bright light or phase microscopy, count the platelets in the large center square of the Cell-VU® CBC ruling (this center square is divided into 100 smaller squares). Multiply the cells counted by 5,000 to obtain the total platelets/cu mm.

For low platelets counts, count the platelets in the large center square as well as the four corner squares of the Cell-VU® CBC ruling. Multiply the cells counted by 1000 to obtain the total platelets/cu mm.

E. Eosinophil count: UNOPETTE test #5877 (1:32 dilution)
Dilute blood using accepted laboratory methods. Disposable dilution preparations such as the UNOPETTE® system #5877 can also be used.

Pipette diluted specimen onto the Cell-VU® CBC slide according to the directions in general preparation. Allow cells to settle for 10 minutes before counting. Isolate and view the Cell-VU® CBC ruling.

Using 100x magnification, count the eosinophils in all 9 squares of the Cell-VU® CBC ruling. Repeat this count in the second chamber. Multiply the number of eosinophils counted by 8.8 to get the total eosinophil count/cu mm.

Note:
1 microliter = 1 cubic millimeter

Cell-VU® CBC for Cell Counts from Spinal/Body Fluids
For spinal/body fluid that is moderately cellular, count the cells in all 9 squares of the Cell-Vu® CBC ruling. Multiply the cells counted by 5.5 to obtain the total cell count/cu mm.

For spinal/body fluid that is bloody (high cellularity) make appropriate dilution as previously described for manual hematology tests.

Note:
1 microliter = 1 cubic millimeter

Pipette one drop (approximately 4 microliters) of undiluted specimen. Place the specimen at the extreme edge of one of the sampling areas (figure F). Two tests can be performed using one Cell-VU® CBC slide.
Make sure the Cell-VU® CBC name on the cover glass is facing the observer as the grid is etched on the reverse side.

Gently lower the cover glass over the specimen so that the edge of the cover glass just covers the sample (figures G and H).

Slide the cover glass into position as shown in figure I. This will eliminate air bubbles from the counting area.

Using Cell-VU® CBC for sperm counts and motility
Isolate and view the central square of the CELL-VU® CBC grid. The central square is divided into 100 smaller boxes each 0.1 x 0.1 mm.

For undiluted semen count, all motile and non-motile sperm within 10 small boxes of this grid. Divide this number by 2. This result is the concentration of sperm in millions/ml.

Suggestions:

For increased accuracy, count all sperm within the entire central square (100 boxes). Multiply the count by 50,000 to obtain the total concentration of sperm/ml.

Sperm can be immobilized by placing a small amount of sample in an appropriate container and then immersing it in hot water for several minutes

If diluted specimen is used, follow the instructions and multiply by the dilution factor.