Sperm Counting ChamberDRM-600
For sperm counts in undiluted specimens. The CELL-VU® Cytometer consists of a standard (3 x 1″), specially-designed glass slide and a coverslip with the counting grid laser-etched into its surface. Disposal of the entire apparatus eliminates cleaning and minimizes clinician exposure to body fluids. Both the slide and coverslip are marked to ensure correct use. The coverslip’s 1 x 1 mm grid is divided into 100, 0.1 mm areas and is thin enough to use under high magnification without special adapters or reticles. The slide consists of two chambers (for dual determinations), each with a depth of 20 µm. This depth is optimal for sperm cells to form in a monolayer, movement is unencumbered, motility can be assessed and counts are made easily.
25 Slides/50 Grid Coverslips
Available for sales in the European Union
DRM600 - CELL-VU Sperm Counting Chamber Instructions for Use
Ensure the CELL-VU® name on the cover glass is facing the observer as the grid is etched on the reverse side.
Place one cover glass onto the slide as shown in Figure A.
The cover glass should cover approximately three quarters of one of the sampling areas, as shown in Figure B.
Mix the sample thoroughly just before pipetting.
Pipette one drop (approximately 4 microliters) of specimen into the edge of the sampling area so that it fills the sampling area by capillary action as shown in Figure C.
Move the cover glass into final position so that the circle on the cover glass is centered within the circle of the sampling area (Figures D and E).
This will eliminate air bubbles from the counting area.
Isolate and view the CELL-VU® grid. This grid is divided into 100 small boxes each 0.1 x 0.1 mm.For undiluted sperm count all motile and non-motile sperm within 10 small boxes of this grid. Divide this number by 2. This result is the concentration of sperm in millions/ml. For specimens with low sperm counts, count all sperm within the entire central square (100 boxes). Multiply the count by 50,000 to obtain the total concentration of sperm/ml.
Count both motile sperm and non-motile sperm within 10 small boxes of the grid. Divide total sperm (motile + non-motile) by the total motile count. Multiply by 100 to establish the percentage of motile sperm.